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DHPLC检测奶牛乳铁蛋白基因点突变方法的研究
Study on the DHPLC Method of Screening Point Mutation of Bovine Lactoferrin Gene
【摘要】 乳房炎是奶牛生产中最普遍、最复杂也是花费最多的疾病,与乳铁蛋白存在一定的相关性。本实验以DHPLC取代SSCP检测bLF基因点突变。结果表明,DHPLC对点突变的检出率达到97%,优于SSCP等方法。
【Abstract】 Mastitis is the most frequent,complex and costly disease in dairy cattle and has a close relationship with the lactoferrin.In this present study,DHPLC is used to screen the point mutation instead of SSCP and the 97% of the accuracy indicates DHPLC is a good method compared with SSCP,et al.
【基金】 国家“十五”科技攻关重大专项(No:2002BA518A12);安徽省教育厅自然科学研究项目(No:2006KJ201B)
- 【文献出处】 安徽科技学院学报 ,Journal of Anhui Science and Technology University , 编辑部邮箱 ,2007年04期
- 【分类号】S823
- 【下载频次】100