【摘要】 [目的]介绍一种将常规方法改进成为一种适用于分离存放时间过久的细条病样本的新方法。[方法]用注射器渗压法将病害标本的研磨液接种水稻叶片形成新鲜病斑后,再按改进的常规方法进行分离纯化。[结果]该新方法分离纯化可直接得到单菌落。[结论]该方法是一种适用于分离存放时间过长的水稻细菌性斑条病菌标本的方法。更多还原

【Abstract】 [Objective] A new method for long store of isolated rice bacterial leaf steak sample was introduced according to the conventional method. [Method] Specimen homogenate was inoculated to the rice leaf by injector osmotic pressure method and then the new and fresh disease lesion appeared. Modified conventional method was adopted for isolation. [Result] Single colony was easily obtained by this isolation method. [Conclusion] This was an efficient method to isolate the pathogen of the rice bacterial leaf steak sample after longtime store.更多还原