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包埋-脱水法常温和常低温保存条斑紫菜自由丝状体

Storage of the Free Living Conchocelis of Porphya Yezoensis Ueda by Encapsulation-Dehydration under Normal and Normal Low Temperature

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【作者】 王欢曹光辉高艳萍王起华

【Author】 Wang Huan Cao Guanghui Gao Yanping Wang Qihua(School of Life Sciences,Liaoning Normal University,Dalian,Liaoning116029,China)

【机构】 辽宁师范大学生命科学学院辽宁师范大学生命科学学院 辽宁大连116029辽宁大连116029

【摘要】 用包埋-脱水法在常温和常低温下保存条斑紫菜自由丝状体,探讨了温度、光(暗)和含水量等因素对存活率的影响。结果表明,条斑紫菜自由丝状体在常温或常低温下都可以保存6个月并保持较高的存活率;其中,4℃(暗)保存的最高存活率达到71.5%;而且保存后的丝状体的生长力达到了保存前的水平。包埋-脱水法操作简单,无需贵重设备,在藻类种质保存中有广阔的应用前景。

【Abstract】 The free living conchocelis of Porphya yezoensis Ueda were preserved in normal temperature and normal low temperature by using encapsulation-dehydration method.The facters influencing the algal viability,such as different temperature,illumination intensities and the water content of beads were studied.The results showed that the conchocelis were stored for 6 months under normal temperature and normal low temperature and the high viability were obtained.,the algae viability reached the most point at 71.5 % in the temperature of 4℃(dark),furthermore,the algae viablity of the conchocelis preserved could reach the normal level.The method of encapsulation-dehydration simplifies the operation procedure and do not need expensive equipments,so it is a potential method for preservation of the algae.

【基金】 国家自然科学基金(30170099,30470184)资助
  • 【文献出处】 安徽农学通报 ,Anhui Agricultural Science Bulletin , 编辑部邮箱 ,2007年23期
  • 【分类号】S917.4
  • 【被引频次】7
  • 【下载频次】94
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