【摘要】 研究了荷叶中黄酮类物质的提取及其对猪油的抗氧化作用.首先,采用单因素实验法确定了从荷叶中提取黄酮类化合物的最佳五因素五水平的选择实验.得出最佳提取条件:以50%的乙醇作提取剂,按1∶25的固液比,温度为40℃,pH值为5.15,提取时间为90 min。其次,研究了提取物对猪油的抗氧化作用.将提取液以不同比例添加到猪油中,恒温并定期用Na2S2O3-I2滴定法测油样的过氧化值,结果表明:荷叶中黄酮类化合物对猪油有明显的抗氧化作用,且黄酮类化合物的添加量在实验剂量范围内与其抗氧化性呈正相关;当添加量为0.5%时,荷叶中黄酮类化合物的抗氧化性能可与BHT相媲美;同时还研究了荷叶提取物与Vc的协同抗氧化作用,结果表明荷叶黄酮类化合物与Vc有较好的增效协同效应。更多还原

【Abstract】 The extraction of bioflavonoid from lotus leaves was studied and the antioxidant activity of the extract towards lard was evaluated.First,the extract conditions were optimized by the single factor experiment,such as extraction solvent density,ratio of solid to liquid of extraction,temperature of extraction,pH of extraction and time of extraction.The optimum conditions of operation were determined as follows:50% alcohol,solid/liquid ratio 1∶25,extracting for 1.5 hours under temperature 40℃ and pH 5.15.Secondly,the antioxidant activity of the extract towards lard was assayed by Na2S2O3-I2 titration Method.The results showed that the lotus leaves of bioflavonoid was directly proportional to the antioxidation.When the concentration of flavones in lard was 0.5%,the antioxidative effect was as good as BHT.Meanwhile,when ascorbic acid was used together with bioflavonoid,ascorbic acid shows stronger synergistic antioxidative activities with Lotus leaves bioflavonoid.更多还原