【摘要】 以10个谷子品种为材料,利用A-PAGE的方法,探索了谷子盐溶蛋白分离的适宜实验条件。通过改变H2O2用量、胶浓度、交联度、电极液pH值、进样量及有无浓缩胶等因素,获得了不同盐溶蛋白酸性电泳图谱,经综合分析,提出了分离谷子盐溶蛋白的适宜A-PAGE电泳体系。具体指标为:1 ml胶液中用1μl 3%H2O2,胶浓度13%,交联度4.0%,电极液pH值3.7,进样量控制在20~30μl之间。在有浓缩胶的条件下,盐溶蛋白分离效果好,电泳图谱清晰,分辨率高。更多还原

【Abstract】 The experiment was conducted to optimize the conditions of A-PAGE for isolating salt soluble proteins of foxtail millet.10 foxtail millet varieties were used for A-PAGE.A number of A-PAGE electropherograms were obtained at different levels of dosage of catalyst,gel concentrations,cross-linkages,injecting volumes,pH of gel buffer,and the use of inspissate gel.The optimal conditions for A-PAGE were raised after deliberate investigation.Detailed criteria were as following: dosage of 3% H2O2 1 μl/ml gel,gel concentration 13%,cross-linkages 4%,pH of gel buffer 3.7,and the injecting volumes between 20 and 30 μl.Furthermore,the use of inspissate gel enhanced the resolution of gel electropherograms.更多还原