【摘要】 背景与目的:用TK6人类淋巴母细胞TK基因突变试验评价葛根素的抗诱变性。材料与方法:设溶剂对照组、5μg/ml甲基磺酸甲酯(MMS)组、5μg/mlMMS分别加葛根素25μg/ml、50μg/ml、100μg/ml的各试验组、5μg/mlMMS+100μg/mlVitC对照组,共6组。按上述分组加入葛根素与MMS处理4h后测定第0d的平板接种效率(PE0)、第3d的平板接种效率(PE3)、细胞悬浮生长率(RSG)和总突变频率(TMF)。结果:100μg/ml葛根素剂量组能显著降低MMS诱导的TK基因突变频率。结论:在本实验条件下,葛根素在较高剂量范围可抑制MMS诱导的TK6细胞TK基因突变,有潜在的开发应用前景。更多还原

【Abstract】 BACKGROUND & AIM:To detect and evaluate the antimutagenicity of puerarin by TK gene mutation test.MATERIALS AND METHODS:The TK6 human lymphoid cells were exposed to puerarin at concentrations of 25,50,100 μg/ml with 5 μg/ml MMS,0.1% DMSO,5 μg/ml MMS and 5 μg/ml MMS+100 μg/ml VitC using simultaneous treatment to determine PE0,PE3,RSG and TMF.RESULTS:Puerarin at concentration 100 μg/ml significantly decreased the TMF induced by MMS.CONCLUSION:Under this experimental condition,puerarin inhibited TK gene mutation on TK6 cell induced by MMS at higher concentration,and has good potential for further development and application.更多还原