节点文献
木香、川木香、土木香的高效液相色谱指纹图谱鉴别
The HPLC fingerprint study of Radix Aucklandiae,Radix Vladimiriae and Radix Inulae
【摘要】 目的:建立鉴别木香、川木香、土木香的方法。方法:Kromasil C18柱(250 mm×4.6 mm,5 μm),甲醇-水梯度洗脱,检测波长为254 nm,流速为1 mL·min-1,柱温为30℃。结果:在选定色谱条件下,建立了稳定可控的高效液相色谱图;应用主成分分析法、系统聚类分析法将药材分成三类,并计算相似度。结论:应用高效液相色谱法鉴别木香、川木香、土木香是可行的。
【Abstract】 To identify Radix Aucklandiae,Radix Vladimiriae and Radix Inulae.Method:The finger- prints were obtained from Waters 2695 instrument,the HPLC was performed on a Kromasil C18(250 mm×4.6 mm, 5μm) analytical column gradient eluted with methanol- water at the flow rate of 1 mL·min-1.The temperature of column was 30℃.The UV detection wavelength was 254 nm.Result:Principal components analysis and clustering analysis were used in the identification of the samples,and the similitude was computed by computer.Conclusion: The method can identify Radix Aucklandiae,Radix Ⅵadimiriae and Radix Inulae.
【Key words】 HPLC fingerprint; Radix Aucklandiae; Radix Vladimiriae; Radix Inulae; clustering analysis; principal components analysis;
- 【文献出处】 药物分析杂志 ,Chinese Journal of Pharmaceutical Analysis , 编辑部邮箱 ,2006年11期
- 【分类号】R284
- 【被引频次】29
- 【下载频次】716