【摘要】 目的:建立了透皮吸收制剂中苦参碱的胶束反相HPLC分析方法。以含量为指标之一考查透皮吸收制剂中苦参碱在不同条件下的稳定性。方法:采用C₁₈柱,流动相为甲醇-3.5%十二烷基磺酸钠水溶液（7:3）,以烟酰胺作内标物,检测波长210 nm。结果:苦参碱在20～120μg·mL^-1范围内呈良好的线性关系（r=0.9998）,平均回收率96.22%,RSD=1.6%。结论:流动相中加入胶束溶液,较不加胶束溶液的流动相,有效的防止了苦参碱的拖尾,增加了选择性,且回收率和精密度高。更多还原

【Abstract】 Objective:To establish the method for examination of matrine in creams with micellar reverst-phase HPLC,in order to study the stability of matrine in creams.Method:A C₁₈-column was used as analytical column, the mobile phase consisted of methanol-3.5% SDS water（7:3）,nicotinamide was used as the internal standard, and at the detection wavelength of 210 nm.Results:The linear range was between 20-20μg·mL^-1.The average recovery was 96.22% ,with RSD of 1.6%.Conclusion:the method for determination of matrine in creams with mi- cellar reverst-phase HPLC was proved to be sensitive and selective.It has higher average recovery and precision.更多还原