【摘要】 目的:建立HPLC指纹图谱方法鉴别广金钱草与金钱草药材;方法:以ODS-C₁₈5μm,250mm×4.6mm色谱柱,水（A） -乙腈（B）为流动相,梯度洗脱方式:0～15min,保持85%A,15→60min,85%A→78%A,流速0.7mL·min^-1,柱温30℃,进样量30μL,检测波长215,270,340nm;结果:广金钱草可得到以异牡荆素为参照的11个指纹峰,金钱草可得到以槲皮素为参照10个指纹峰进行比较;结论:通过指纹图谱方式,可针对性地鉴别广金钱草和金钱草。更多还原

【Abstract】 Objective:Established a fingerprint method to identify the Herba Desmodii Styracifolii and Herba Lysi- machiae.Methods:The chromatographic column of ODS-C₁₈5μm,250 mm×4.6 mm was used with water-ace- tonitrile as mobile phase by 0.7 ml·min^-1flow rate under 215,270,340 nm wavelength at 30℃column tempera- ture.The gradient elution is 0-15 min,keep 85% water,15-60 min ,85% water→78% water.The injection volume was 30μL.Results:It compared 11 peaks from Herba Desmodii Styracifolii fingerprint and 10peaks from Herba Ly- simac hiae fingerprint with isovitexin and quercetin as standard respectively.Conclusion:The method can identify the Herba Desmodii Styracifolii and Herba Lysimachiae with HPLC fingerprint.更多还原