【摘要】 目的:研究鲎试剂的平衡浊度值的意义和作用。方法:在 BET-32C 细菌内毒素测定仪上,采用不同的鲎试剂浓度与内毒素进行反应,根据其反应过程中浊度值的变化,获得相应的平衡浊度值和特征反应时间 t_0.077,研究二者之间关系。其中t_0.077表示浊度值达到0.077的时间,也是反应体系形成凝胶所需的时间。结果:（1）4个厂家相同灵敏度的19个鲎试剂,其t_0.077的平均 RSD 为25%左右,平衡浊度值为0.087～0.27,主要在0.10～0.20间,差别较大;（2）鲎试剂浓度影响着 t_0.077的大小;（3）平衡浊度值与鲎试剂浓度呈线性关系,R²>0.90;浓度为0.1～1.0 EU·mL^-1的细菌内毒素与鲎试剂反应,其平衡浊度值为0.136±0.017;（4）平衡浊度值的变化影响 t_0.077值的大小,在试验范围内,t_0.077有最小值;对 A、B 2个厂家的6种鲎试剂进行了实验,当平衡浊度值为0.10～0.15时,t_0.077最小。结论:鲎试剂的平衡浊度值与凝胶形成的快慢有关,建议通过控制平衡浊度值以充分发挥鲎试剂的效率。更多还原

【Abstract】 Objective:To study the meaning of the equilibrium turbidimetric value(OD_equi) and its usage in gel- clot assay.Methods:Endotoxins were reacted with tachypleus amebocyte lysate（TAL）of different concentrations in BET-32C bacterial endotoxin tester.Then the OD_equi,characteristic reactive time t_0.077 were read and the relation- ship between them was investigated.The t_0.077,which was defined as the time spent when the turbidimetric value of the mixture reached to 0.077,indicates the time needed when the gel-clot formed in the mixture.Results:（1） The mean RSD of t_0.077 of the same sensitivity TALs from four manufacturers was about 25% and their OD_equi ranged from 0.087 to 0.27,mainly from 0.10 to 0.20;（2）The t_0.077 were changed with the TAL concentrations;（3）The OD_equi were proportioned to the TAL concentrations.The OD_equi of the tested TAL was 0.136±0.017 when 0.1- 1.0 EU·mL^-1 endotoxin were used;（4）The minimum t_0.077 were observed when the OD_equi were from 0.10 to 0.15 for TALs of manufacturer A and B.Conclusion:The equilibrium turbidimetric values,read by the dynamic tur- bidimetric method in bacterial endotoxin tester,were proportioned to the TAL concentrations and less affected by the endotoxin concentration.The minimum t_0.077 was observed when the OD_equi changed.更多还原