【摘要】 目的:建立血浆中强力霉素检测的固相萃取-高效液相色谱法（SPE-HPLC）。方法:血浆样品经 C₁₈固相萃取小柱提取后进样测定,色谱柱为 Kromasil C₁₈（250 mm×4.6 mm,5μm）,柱温30℃,流动相为0.01mol·L^-1草酸溶液-乙腈-甲醇（55:25:20）,流速为0,8 mL·min^-1,检测波长为350 nm,进样量20μL。结果:血浆强力霉素浓度在0.1～5.0μg·mL^-1范围内线性关系良好（r=0.9996）;最低检测限为0.1μg·mL^-1;样品的平均回收率为93.9%～104%;日内和日间精密度均小于4.81%。结论:方法简便、准确可靠,适用于血浆强力霉素浓度测定及其药代动力学研究。更多还原

【Abstract】 Objective:To develop a solid phase extraction and high performance liquid chromatography（SPE- HPLC）method for the determination of doxycycline in pig plasma.Methods:Plasma samples were prepared with C₁₈ solid phase extraction column.The chromatographic separation was performed on a Kromasil C₁₈ column（250 mm ×4.6 mm,5 μm）at colunm temperature of 30 ℃.The mobile phase,a mixture of 0.01 mol·L^-1 oxalic acid- acetonitrile-methanol（55:25:20）,was delivered at a flow rate of 0.8 mL·min^-1.The detection wavelength was 350 nm.Results:Excellent line relationship was obtained in the range of 0.1-5.0 μg·mL^-1（r=0.9996）.The determination limit of doxycycline was 0.1 μg·mL^-1.The average recovery rate was 93.9%-104%.The intra- day and inter-day RSDs were less than 4.81%.Conclusion:The method is simple,accurate and can be used to determine the doxycycline concentration in plasma and for study of its pharmacokinetics.更多还原