【摘要】 目的:建立测定罗红霉素血药浓度的 HPLC 法。方法:色谱柱为 ODS 柱（150 mm×4.6 mm,5 μm）;流动相为乙腈-甲醇-20 mmol·L^-1磷酸二氢钠溶液（40:20:40,pH 4.7）;流速1.0 mL·min^-1;检测波长为210 nm;柱温:35℃。结果:线性范围为0.2～32 mg·L^-1;最低检出浓度为0.06 mg·L^-1;罗红霉素保留时间为5.4 min;平均回收率97.4%;日内 RSD 小于2.5%,日间 RSD 小于3.0%。结论:本法检测罗红霉素血药浓度具有快速简便、灵敏准确等优点。更多还原

【Abstract】 Objective:To develop an HPLC method for the determination of roxithromycin in human serum.Meth- ods:The analytical column was C₁₈（150 mm×4.6 mm,5 μm）;the mobile phase consisted of a mixture of acetoni- trile-methanol-20 mmol·L^-1 NaH₂ PO₄ solution（40:20:40,pH 4.7）;pumped at 1.0 mL·min^-1 through the column;the wavelength was set at 210 nm and kept at 35 ℃.Results:The standard curve was linear in the concen- tration range of 0.2-32 mg·L^-1;the detection limit in human serum was 0.06 mg·L^-1;retention times for rox- ithromycin was 5.4 min:the average method recovery was 97.4%;the intra-day and inter-day RSD were less than 2.5% and 3.0%,respectively.Conclusion:This is a rapid,sensitive and accurate method for the determina- tion of roxithromycin in human serum.更多还原