【摘要】 目的:建立黄芪注射液中2个黄烷类化合物的高效液相色谱含量测定方法。方法:采用 Agilent ZORBAX-SB-C₁₈分析柱（250mm×4.6mm,5μm）;流动相为甲醇-0.1%磷酸水溶液（43:57）;流速:1mL·min^-1;检测波长:277nm;最佳固相萃取洗脱条件减少了定量分析的杂质干扰。结果:9,10-二甲氧基紫檀烷-3-O-β-D-吡喃葡萄糖苷（HQ-1）,线性范围为0.0888～1.4208μg,相关系数为0.9999;平均回收率为104.4%,RSD=0.4%。2′-羟基-3′,4′-二甲氧基异黄烷-7-O-β-D-吡喃葡萄糖苷（HQ-2）,线性范围为0.0555～0.888μg,相关系数为0.9999;平均回收率为97.72%,RSD=1.4%。结论:该方法简便、准确、快速,结果可靠,为今后对黄芪注射液的质量控制研究提供了补充依据。更多还原

【Abstract】 Objective:To develope a method for the determination of 9,10-dimethoxypterocarpan-3-O-β-D -glucopyranoside（HQ-1）and 2′-hydroxy-3′,4′-dimethoxyisoflavan-7-O-β-D-gluco-pyranoside （HQ-2）in Huangqi injection using solid phase extraction coupled with RP-HPLC.Method:The Agilent ZORB- AX SB-C₁₈ column（250 mm×4.6 mm,5 μm）was used.The mobile phase was methanol-0.1% phosphoric acid（43:57）and the detection wavelength was 277 nm.The optimum conditions for extraction was studied in de- tail.The extraction time was shorter while the extraction efficiency was higher.The quantification could be done.Re- suits:The method had good linear relationship within range 0.0888-1.4208 μg,0.0555-0.888 μg of HQ-1 and HQ-2.The average recoveries were 104.4%（HQ-1）and 97.72%（HQ-2）respectively.Conclusion: The method is convenient,rapid,accurate and reliable,providing a basis for quality analysis of the Huangqi injec- tion.更多还原