【摘要】 目的:寻找金银花中的抗呼吸道病毒感染的成分,并进行定量研究。方法:采用柱层析等方法分离、细胞病变法进行抗病毒作用的研究,采用 RP-HPLC 方法进行定量方法的研究。色谱系统:色谱柱为 HiQ Sil C₁₈色谱柱（250mm×4.6mm,5μm）,流动相为乙腈-水（15:85）,柱温:室温,检测波长240nm,流速1mL·min^-1,进样量10μL。结果:分离得到了10种环烯醚萜苷类化合物,确认环烯醚萜苷类成分具有中等强度的抗呼吸道病毒感染的作用,并采用RP—HPLC方法测定了金银花药材中的4种环烯醚萜苷的含量。结论:环烯醚萜苷类成分为金银花中的有效成分,定量方法准确,重复性好。更多还原

【Abstract】 Objective:To search for the antiviral components from the flowers of Lonicera japonica ThuMb.and de- termine the contents of iridoids in Flos Lonicerae Japonicae.Methods:To isolate components with different chroma- tographic method and test the antiviral activities in CPE methods.The contents were determined by RP-HPLC method.The separation was carried out on a HiQ Sil C₁₈（250 mm×4.6 mm,5 μm）at room temperature with mo- bile phase of acetonitrile and water（15:85）.The detection wavelength was set at 240 nm.The flow rate w.s 1.0 mL ·min^-1.The injected volume was 10 μL.Results:10 iridoids were isolated from Flos Lonicerae Japonicae.These iridoids showed moderate antivial activities against respiratory viral infections.The contents of 4 iridoids were deter- mined by RP-HPLC.Conclusion:The iridoids are effective components and the method is simple and accurate.更多还原