【摘要】 目的:建立 HPLC 法拆分了谷氨酸（Glu）和天门冬氨酸（Asp）对映体的方法。方法:本文利用邻苯二甲醛（OPA）柱前衍生,以β-环糊精（β-CD）为手性添加剂,乙腈-缓冲溶液(0.02mol·L^-1醋酸钠:三乙胺=100:0.018,)为流动相,检测波长为330nm。考察了不同色谱柱、缓冲溶液 pH、β-环糊精浓度、流动相比例和柱温对分离的影响。优化的色谱条件为:色谱柱为 Hypersil AA-ODS,分离温度40℃,流动相为乙腈-缓冲溶液(0.02mol·L^-1醋酸钠:三乙胺=100:0.018,用2%醋酸调pH 7.2,其中含6mmol·L^-1β-CD)=1:99。结果:在优化的色谱条件下,上述两种氨基酸对映体均得到基线分离。结论:所建立的方法简便、快速、分离度高。更多还原

【Abstract】 Objective:To establish a method for the chiral separations of glutamic acid（Glu）and aspartic acid （Asp）by high performance liquid chromatography（HPLC）.Method:The amino acids were derivatized with o- phthaladehyde（OPA）before separations.β-CD was used as a chiral mobile phase additive.The mobile phase was acetonitrile-buffer solution [0.02 mol·L^-1NaAc-（CH₃CH₂）₃ N=100:0.018].The detective wavelength was 330 nm.The influences of colums,the composition of mobile phase,pH,the concentration of β-CD and colum temperature on the enantiomerie separations of Glu and Asp were investigated.The optimized chromatographic con- ditions for the separations were at 40℃ on a Hypersil AA-ODS colum and the mobile phase was acetonitrile- buffer solution [0.02 mol·L^-1NaAc-（CH₃ CH₂）₃ N=100:0.018,adjusting to pH=7.2 with 2% HAc and con- taining 6 mmol·L^-1β-CD]（1:99）at a flow rate of 0.45 mL·min^-1.Results:Under the chromatographic con- ditions,baseline separations of these enantiomers could be obtained.Conclusion:The method was simple,rapid with high resolutions.更多还原