【摘要】 目的:用 HPLC 梯度洗脱法同时测定蛇胆中牛磺胆酸（TCA）、牛磺鹅去氧胆酸（TCDCA）、牛磺去氧胆酸（TDCA）、甘氨胆酸（GCA）4种结合型胆汁酸的含量。方法:用美国 Zorbax Extend C₁₈柱,乙腈-0.15%磷酸氢二钠溶液梯度洗脱0～20min（15:85～40:60）。流速1.0mL·min^-1,检测波长203nm。结果:TCA、TCDCA、TDCA、GCA 在进样量为2～39μg范围内线性关系良好。该方法回收率 TCA 为99.5%（RSD=0.5%）,TCDCA 为100.3%（RSD=1.8%）,TDCA 为100.0%（RSD=1.8%）,GCA 为98.9%（RSD=2.2%）。结论:HPLC 梯度法能将蛇胆中结合型胆汁酸很好分离,方法简单,准确。更多还原

【Abstract】 Objective:An HPLC gradient elution was studied for the determination of TCA,TCDCA,TDCA,GCA in snake gall MethodS:C₁₈column was used with a mobile phase of acetontrile-0.05% Na₂HPO₄,0-20 min of （15:80-40:60）gradient elution post time was 20 min,the wavelenth of detected was set at 203 nm.Results:TCA ,TCDCA,TDCA,GCA calibration curves were linear at the range of 2-39 μg.The average recoveryies rate TCA was 99.5 %（RSD=0.5 %）,TCDCA was 100.3 %（RSD=1.8 %）,TDCA was 100.0%（RSD=1.8 %）,GCA was 98.9%（RSD=2.2%）respectively.Conclusion:The method is simple and accurate.The results show that the method can be used to control quality of Snake gall.更多还原