【摘要】 目的:采用反相高效液相色谱法测定反式白藜芦醇及其有关物质。方法:以岛津 C₁₈柱（5μm,150mm×4.6mm）为分析柱,甲醇-0.1mol·L^-1乙酸（40:60）为流动相,流速1.0mL·min^-1,检测波长为305nm。结果:标准曲线线性范围为12～28μg·mL^-1（r=0.9999）。最低检测限和定量限分别为0.09和0.29ng。在所建立的色谱条件下,反式白藜芦醇与其异构体杂质的分离度为7.20,与酸、碱、光照降解产物也有良好的分离效果。反式白藜芦醇对温度（60℃）、光线（4000lx）及湿度（RH%:75%及92.5%）敏感。结论:方法简便、准确、灵敏,可用于白藜芦醇的常规分析。更多还原

【Abstract】 Objective:To develop an RP-HPLC method to determine trans-resveratrol and its related sub- stances.Methods :The C₁₈column（5μm,150mm×4.6mm ）was used as analytical column.Mobile phase con- sisted of methanol-0.1 mol· L^-1acetic acid（40:60）,flow rate was 1.0 mL·min^-1.The detection wavelength was set at 305 nm.Results:The standard curve was linear in the concentration range from 12 to 28μg·mL^-1for trans-resveratrol（r=0.9999）.The limit of detection and quantitation for the assay were 0.09 and 0.29ng,re- spectively.Under the established conditions,the resolution between trans-resveratrol and its isomer was 7.20 and trans-resveratrol could also be well separated from its degradation products from acidic and basic hydrolysis and light exposure.Trans-resveratrol was sensitive to high temperature（60℃）,light（4000 lx）and humidity （RH%:75%and 92.5%）.Conclusion:The method is simple,accurate and sensitive,and can be used for the routine analysis of resveratrol.更多还原