【摘要】 目的:检测细辛药材不同用药部位。肾毒性成分马兜铃酸 A 的含量。方法:高效液相色谱方法,采用 Agilent Zorbax SB-C₁₈色谱柱（4.6 mm×250 mm,5μm）,以乙腈-1%冰醋酸水溶液（39:61）为流动相,流速为0.8 mL·min^-1,检测波长为317 nm,分别测定细辛全草和根、芦头、叶柄、叶片、花5个部分马兜铃酸 A 的含量。结果:高效液相色谱方法的回收率为98.7%,马兜铃酸 A 进样量在5.2～68ng 范围内线性关系良好;细辛全草含马兜铃酸 A 16.2μg·g^-1,根和芦头中含微量马兜铃酸 A,叶柄和叶片中有较多马兜铃酸 A,花中含量最高。结论:细辛地上部分和地下部分马兜铃酸 A 含量差别很大。更多还原

【Abstract】 Objective:To determine the nephropathy-causing substance,aristolochic acid A,in different parts of As- arum sieboldii Miq.Methods:An HPLC method was established with a Agilent Zorbax SB-C₁₈ column（4.6 mm× 250 mm,5 μm）,using a mobile phase of acetonitrile-0.1%acetic acid solution （39:61）,a flow rate of 0.8 mL·min^-1,a detection wavelength of 317 nm to determine the contents of aristolochic acid A in Asarum siebol- dii Miq.’s root,roothead,petiole,lamina,flower and the whole plant.Results:The average recovery rate was 98.7%,and there was a good linearity from 5.2 ng to 68 ng.The concentration of aristolochic acid A in the whole plant was 16.2 μg·g^-1,and there were very little in the root and roothead,and more in petiole and lamina,and the most in flower.Conclusion:The underground parts of Asarum sieboldii Miq.have much aristolochic acid A while the root has very little,and the root is more suitable to be used in medicine.更多还原