【摘要】 目的:建立高效液相色谱法测定金银花样品中绿原酸、当药苷和忍冬苷的含量。方法:采用 YMC-Pack ODS-C₁₈色谱柱（150mm×4.6mm,5μm）,以乙腈（A）-1%冰醋酸水溶液（B）为流动相梯度洗脱,多波长检测绘制金银花药材中绿原酸、当药苷和忍冬苷的 HPLC 图谱。流动相梯度:0min（5%A）→13min（10%A）→30min（25%A）。流速:1.0mL·min^-1。波长:0～17min,用325nm 检测绿原酸;17～22min,用237nm 检测当药苷;22～25min 用245nm 检测忍冬苷。柱温:20℃。进样量:15μL。结果:绿原酸、当药苷、忍冬苷3个化合物在测定的范围内均表现出良好的线性（r>0.9999）,方法的回收率在101.9%～102.3%之间。结论:该方法操作简便、分离效果好、灵敏度高、重复性好,为较全面控制金银花药材的质量提供了一种可靠的方法。更多还原

【Abstract】 Objective:To establish an HPLC method for the determination of chlorogenic acid,sweroside and loniceraside in Flos Lonicerae.Methods:An HPLC method was applied with a YMC-Pack ODS-C₁₈（150mm× 4.6mm,5μm）column by a gradient elution using （A）acetonitrile-（B）water （1% HAc） as the mobile phase 0 rain （5% A）→13 min（10% A）→30 min （25% A）.The flow rate was 1.0 mL·min^-1.The detection was set at UV 325 nm（0→17min） to detecte chlorogenic acid;UV 237 nm（17→22min） to detecte sweroside;UV 245 nm （22→25 min） to deteete loniceraside respectively.The column temperature was 20℃,and injection volume was 15μL.Results:The recovery of the method was in the range of 101.9%～102.3%,and the compounds showed good linearity （r>0.9999） in a detected concentration range.Conclusion:The method is simple,sensi- tive,accurate and reproducible,it can be used to control the quality of Flos Lonicerae.更多还原