【摘要】 目的:建立河北道地药材北柴胡的 HPLC-ELSD 指纹图谱分析方法,以提高柴胡药材质量标准。方法:采用 HPLC-ELSD 法测定了道地北柴胡等21个不同来源的柴胡样品。色谱条件:C₁₈柱,乙腈-水为流动相进行梯度洗脱,ELSD 检测器,流速1.0mL·min^-1,柱温30℃。结果:建立了北柴胡 HPLC-ELSD 指纹图谱共有模式,并对不同产地柴胡药材进行了相似度比较。结论:色谱指纹图谱分析法能简便、快速地鉴别和区分不同来源的柴胡药材,为全面控制柴胡药材的质量提供了依据。更多还原

【Abstract】 Objective:To establish a fingerprint analysis method for identification of Bupleurum chinense DC.of He- bei province and quality control of Radix Bupleuri.Methods:The HPLC-ELSD fingerprints of Radix Bupleuri from different places were obtained from Waters 1525 instruments.The HPLC separation was performed on a C₁₈ana- lytical column gradient eluted with a mixture consisting of acetonitrile and water at a flow rate of 1.0 mL·min^-1 with ELSD detector.The temperature of column was 30℃.Results:The mutual mode of HPLC-ELSD fingerprints were set up,and the similar degrees to the crude drugs collected in different areas were compared.Conclusion:It is simple and quick to verity Bupleurum chinense from different sources with the method,and can be used as a quality control item for Radix Bupleuri.更多还原