【摘要】 目的:研究了金属离子对多西环素（DC）体系荧光光谱的影响。方法:用日立 F-3010型荧光分光光度计测定,在表面活性剂十二烷基硫酸钠（SDS）存在下,DC 与Zn²⁺形成络合物而使 DC 荧光增强,其λ_ex=398nm,λ_em=520nm。测定时最佳pH 为9.85。结果:DC 浓度在1.25～9.5μg·mL^-1范围内与荧光强度呈线性关系,相关系数0.9943,检测限为0.014μg·mL^-1。结论:该方法应用到实际样品的测定,结果满意。更多还原

【Abstract】 Objective:To study the influence of metal ions on the fluorescence spectra of doxycycline（DC）. Methods:Hitachi F-3010 model spectrofluorimeter.With the presence of SDS,DC react with Zn²⁺and form a complex,the fluoresence intensity was added with λ_ex=398 nm,λ_em=520 nm.The fluorescence intensity of DC reached maxinum at pH=9.85.Results:The fluorescence intensity was linearly related to the DC concentration in the range of 1.25-9.5μg·mL^-1with correlation coefficient 0.9943.The determination limit was 0.014μg· mL^-1.Conclusion:It was satisfactorily applied into the determination of DC in pharmaceutical preparations.更多还原