【摘要】 目的:建立高效液相色谱测定竹红菌中竹红菌甲素含量的方法。方法:采用 Zorbax Extend-C₁₈柱（4.6mm×150mm,5μm）,以甲醇与水（83:17,体积比）为流动相,流速1mL·min^-1。荧光检测器激发波长为410nm,发射波长610nm。结果:竹红菌甲素在25～250μg·mL^-1浓度范围内呈良好的线性关系（r=0.9999）,平均回收率（n=6）为100.3%。结论:本分析方法简便快速,灵敏准确,可用于竹红菌中竹红菌甲素含量的测定。更多还原

【Abstract】 Objective:To establish an HPLC method for the determination of hypocrellin A（HA）in Hypocrella bambusae（B.et Br.）Sacc samples.Method:A Zorbax Extend-C₁₈column（4.6 mm×150 mm,5μm）and a mobile phase consisting of methanol-water（83:17 by volume）were employed.The HPLC conditions were:flow- ing rate 1 mL·min^-1,injection aliquot 10μL,column temperature 30℃,fluorescence detection excitation wave- length 410 nm and emission wavelength 610 nm.Results:HA showed good linearity（r=0.9999）in the range of 25-250μg·mL^-1.The average recovery（n=6）was 100.3 %.Conclusion:The HPLC-FLD method is sim- ple,sensitive,accurate and rapid for the determination of HA in Hypocrella bambusae（B.et Br.）Sacc.更多还原