【摘要】 目的:建立虎杖药材指纹图谱分析方法。方法:采用 Agilent^3DCE 高效毛细管电泳仪,未涂渍熔融石英毛细管（75μm×48.5cm,有效长度40.0cm）,压力进样5.25Pa×3s,运行缓冲液为10mmol.L^-1硼酸钠溶液-甲醇（1:1）,检测波长为220nm,运行电压30kV,温度25℃,内标为厚朴酚。结果:确定了虎杖药材甲醇提取部分12个共有峰,不同产地虎杖样品指纹图谱与对照指纹图谱相似度系数均在0.9以上,可用于虎杖药材的定性鉴别。结论:方法准确可靠,为提高虎杖药材质量控制标准提供参考。更多还原

【Abstract】 Objective:To establish the fingerprint of Rhizoma Polygoni Cuspidati by HPCE.Method:Untreated fused silica capillary（48.5cm×75μm,effective length is40cm）was used.Pressure injection of 5.25Pa×3s was employed,and the temperature was 25℃.The detection wavelength was set at220nm.The samples were ana- lyzed with 10mmol·L^-1sodium borate-methanol（1:1）running buffer at 30kV voltage.The internal standard substance was maguolol.Result:12common peaks were found in the fingerprints of Rhizoma Polygoni Cuspidati and good similarities with correlation coefficients higher than0. 9were found in fingerprints between the herbs from dif- ferent sources and the standard fingerprint,which can be utilized for the identification of Rhizoma Polygoni Cuspi- dati.Conclusion:The method is accurate and reproducible,providing a new reference for quality control of Rhizo- ma Polygoni Cuspidati.更多还原