【摘要】 对卷枝毛霉(Mucorcircinelloides)3.2208进行紫外诱变,采用苏丹黑染色和TTC酶活法进行初筛,摇瓶发酵测定相关指标进行复筛,获得1株γ亚麻酸(γlinolenicacid,GLA)产率比出发菌株显著提高的突变株M3,经培养,M3菌体细胞收率为15.20g/L,油脂含量为20.10%,油脂产率为3.65g/L,GLA含量占菌体油脂总脂肪酸的17.28%,产率达630.72mg/L.更多还原

【Abstract】 <Abstrcat> Mucor circinelloides 3.2208 was mutagenized with UV, and screened the first time by Sudan Black staining and TTC enzyme activity testing first, and then screened once again by testing the related indexes in flask shaking culture. A mutant M₃ with higher GLA yield was obtained. Dry cell weight and lipid yield of M₃ reached 15.20 g/L and 3.65 g/L respectively, and GLA yield of M₃ reached 630.72 g/L, which was 17.28% of the total lipids.更多还原