【摘要】 建立了一种利用反相高效液相色谱-蒸发光散射检测、通过梯度洗脱测定油脂中脂肪酸、单甘酯、二甘酯与三甘酯含量的方法,色谱柱是C18反相柱,流动相为乙腈(0.05%醋酸V/V)-二氯甲烷,流速1.0 mL/min;蒸发光散射检测器漂移管温度70℃,载气流速1.7 L/min。各物质色谱峰面积的自然对数与浓度的自然对数呈良好的线性关系,精密度RSD为0.43%~2.75%,最低检测限为0.02~0.04μg,回收率测定值为93.2%~104.5%。该方法不仅可以准确、快速地定量、定性分析各类混合脂肪酸甘油酯和游离脂肪酸,而且还可以分离具有立体异构的单甘酯和二甘酯。更多还原

【Abstract】 A method for the determination of mono-,di-,and triglycerides by a binary gradient HPLC with ELSD was established.Chromatographic analysis was carried out on a Alltima C₁₈ column（4.6 mm×250 mm,5 μm） with methylene chlorideacetonitile plus acetic acid（0.05%,V/V） as the mobile phase.The flow rate was 1.0 mL/min.The drift tube temperature and nitrogen carrier gas flow rate of ELSD were set at 70℃ and 1.7 L/min respectively.The correlation coefficient was good with R≥0.9986.The detection limit was from 0.02 μg to 0.04 μg and the average recovery was 93.2% to 104.5%.The method was simple,rapid and accurate.The monoglyceride,diglyceride,triglyceride and free fatty acid can be separated by the method,and the method can be also used to separate the positional isomers of mono-and diglycerides in addition to the mixed di-and triglycerides.更多还原