【摘要】 采用固定化木瓜蛋白酶水解大豆分离蛋白,对大豆分离蛋白的最佳预处理温度进行了探讨,并对制备大豆肽的工艺条件进行了正交实验。结果表明:大豆蛋白的最佳预处理温度为90℃;在底物浓度2.7mg/mL、温度55℃、pH7.8、流速0.6mL/min的条件下,利用固定化木瓜蛋白酶制备大豆肽,酶解液中的可溶性蛋白含量为1.384mg/mL,水解度43.65%;影响酶水解反应的因素主次顺序为:pH>温度>底物浓度>流速。更多还原

【Abstract】 The hydrolysis of soybean protein isolate with immobilized papain for making soy peptide was studied.The optimum process parameters for the hydrolysis were presented as follows:the pretreatment temperature of soybean protein isolate 90℃,hydrolysis temperature 55℃,pH7.8,substrate concentration 2.7 mg/mL,flow rate 0.6 mL/min.Under the best conditions,the hydrolysis degree of soybean isolated protein was 43.65%,the content of soy peptide in hydrolysis product was 1.384 mg/mL.The effect sequence of reaction was hydrolysis temperature,pH, substrate concentration,flow rate of soybean protein isolate.更多还原