【摘要】 目的探讨苦参碱对刀豆蛋白A(ConA)所致肝损伤的防治作用。方法48只NIH小鼠随机分为正常对照组、模型组、苦参碱25mg/kg组、苦参碱12.5mg/kg组和联苯双酯治疗组。除正常对照组外,其他组于实验首日静脉注射ConA20mg/kg,苦参碱两个剂量组均采用尾静脉注射给药,联苯双酯组按150mg/kg灌胃口服,每天1次,连续3d,末次给药后4h,再次静脉注射ConA20mg/kg,8h后检测血浆ALT活性及IFN-γ和TNF-α含量,观察肝组织病理学变化。结果苦参碱25mg/kg组、12.5mg/kg组小鼠ALT活性及IFN-γ和TNF-α含量均明显低于模型组,有显著性差异(P<0.01);苦参碱两个剂量组肝脏病理改变明显减轻。结论苦参碱对ConA所致小鼠肝损伤具有较好的防治作用,抑制T淋巴细胞活化和IFN-γ、TNF-α的释放可能是其主要的作用机制。更多还原

【Abstract】 Objective To investigate the protective effec ts of matrine on liver injury in mice i nduced by concanavalin A(Con A).Methods Forty -eight NIH mice were randomly d ivided into 4groups:normal control group(group A),mod-el group(group B ),matrine 25mg /kg group(group C ),matrine 12.5mg /kg group(group D)and bifendate group(group E ).Excepts those in group A ,mice in oth er groups were injected with Con A 20mg /kg via the tail vein at the first day of experiment.Group C and g roup D were injected with matrine at t he dosage of 25mg /kg and 12.5mg /kg via the tail vein respectively.Group E w ere administered with bifendate by g astric perfusion at the dose of 150mg /kg.All the drugs were given one time a day for 3days continuously.In the 4th hour afte r the last administration,mice were injected with Con A again at the same dosage.Pl asma aminotransferase(ALT)activity,interferon -gamma (IFN -γ)and tumor necrosis factor alpha(TNF -α)contents were measured in the 8th hou r after injection of Con A,and histop athological examination was also performed for l iver tissue.Results ALT activity,IFN -γand TNF -αcontents in groups C and D were obviously lower than those in group B(P<0.01);hepatic histopathological changes were alleviated in both groups C and D.Conclusion Matrine has a good protective effect on liver injury induced by Con A in mic e;its therapeutic mecha-nism may be related to the inhibition of the activation of T -lymphocytes a nd the release of IFN -γand TNF -α.更多还原