【摘要】 目的:建立病毒清口服液中绿原酸的含量测定方法。方法:色谱柱:Lichrospher C18(4.6×200 mm,5μm),流动相: 甲醇:0.2 mol·L-1磷酸二氢钠溶液(用磷酸调节PH至3.2)(25:75),流速0.8 ml/min,检测波长327 nm。结果:绿原酸在0.15 ～0.75μg范围内呈现良好的线性关系,r=0.999 6,平均回收率为97.64%,RSD=1.2%。结论:本方法简便、快速、准确,分离效果好。更多还原

【Abstract】 Objective: To establish a simple sensitive HPLC method for determining chlorogenic acid in Bingduqing oral. Method: Chlorogenic acid was separated on Lichrospher C18 column(4.6×200 mm,5 μm)with a mobile phase of methanol:0. 2 mol·L-1 sodium dihydrogen phosphate solution (adjusted acidity to 3. 2 with phosphoric acid) (25: 75 ) and detected at 327 nm with a flow rate of 0. 8 ml·min-1. Result: The linear range was within 0. 15-0.75 μg, r = 0. 999 6. The average recovery was 98.19% ,RSD=1.3%. Conclusion: The method is simple ,rapid, and reliable as well as good separating degree.更多还原