【摘要】 目的:采用高效液相法测定贯叶连翘中的金丝桃素、芦丁和槲皮素的含量方法。方法:色谱柱为SymmtryC18(4.6mm×250mm,5μm),检测波长分别为588nm,359nm,流动相分别为甲醇-乙腈-1.0%磷酸二氢钠溶液(340∶12∶7)和甲醇-水(40∶60)。结果:线性范围分别在0.02704~0.1352μg和0.264~1.32μg,0.052~0.260μg内良好,回收率分别为98.39%(n=3)、98.56%(n=3)和98.85%(n=3)。RSD分别为0.78%和1.01%和1.02%。结论:该法简便,精密度高,结果准确可靠。更多还原

【Abstract】 Objective: To determine hypericin, rutin and quercertin, which were active constituents, in Hypericum perforatum.L by HPLC . Method: Hypericin, rutin,and quercertin were separated by Symmetry C 18 column（ 4.6 mm×250 mm,5 μl） column with methanol-acetonitile- 1.0% sodium dihydrogen phosphatc （340[DK7]∶12∶7） and methanol-H₂O（40∶60） as mobile phases the detecting ware length were respectively 588 nm and 359 nm. Result: The linear ranges of hypericin, rutin, and quercertin were within 0.02704 0.1352 μg, 0.264 1.32 μg , and 0.052 0.26 μg, respectively. The recoveries were respectively 98.39%（n=3） 98.56%（n=3）, and 98.85%（n=3）,RSD were respectively 0.78%, 1.01%, and 1.02%. Conclusion: The method is simple, accurate, and reliable.更多还原