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心宁胶囊中人参皂苷Rg1的HPLC测定

Determination the Content of Ginsenosides Rg, in Xinning Capules by HPLC

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【作者】 王隶书程东岩李阳

【Author】 Wang Lishu, Cheng Dongyan, Li Yang ( Academy of Traditional Chinese Medicine and Materia Medica of Jilin Province, Changchun 130021, P. R.China)

【机构】 吉林省中医中药研究院吉林工大医院 长春130021长春130021

【摘要】 目的:建立心宁胶囊中人参皂苷Rg1的含量测定方法。方法:HPLC法,流动相:乙腈-0.4%磷酸水溶液(20.5:79.5)为流动相;检测波长为203nm;柱温40℃。结果:人参皂苷Rg1线性范围为0.392μg-4.704μg,平均回收率99.8%,RSD为2.23%。结论:方法可靠,简单可行,为控制心宁胶囊的内在质量提供了科学依据。

【Abstract】 Objective: To establish a method for determining Ginsenosides Rg1 in Xinning capules. Method: HPLC was used with mobile phase: acetonitrile -0. 4% phosphoric acid solution(20. 5: 79. 5) ,the detecting wavelength was at 203 nm. Result: The standard curve was linear within a range of 0. 392-4. 704 μg. The average recovery and RSD were 99. 8% and 2. 23% , respectively. Conclusion : This method is accurate and reliable, and can provide a scientic foundation for controlling the quality of Xinning capules.

【关键词】 心宁胶囊人参皂苷Rg1高效液相色谱法
【Key words】 Xinning capulesGinsenosides Rg1HPLC
  • 【分类号】R286
  • 【被引频次】4
  • 【下载频次】50
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