【摘要】 目的:通过观察滋阴补肾法对卵巢切除所致骨质疏松大鼠骨髓基质细胞IL1和成骨细胞IL6表达的影响,探讨其治疗骨质疏松症的机理。方法:将大鼠随机分为5组,即空白对照组、假手术组、模型组、雌激素组和补肾组。摘除大鼠双侧卵巢3个月后,灌胃给予左归丸,共给药3个月。采用骨组织形态计量学的方法对大鼠胫骨不脱钙骨切片进行形态计量;采用免疫组化和原位杂交的方法分别检测骨髓基质细胞IL1和成骨细胞IL6蛋白和mRNA的表达。结果:与模型组比较,补肾组大鼠胫骨TBV%显著增高,TRS%以及TFS%、MAR、OSW和mAR均明显降低;同时,其骨髓基质细胞IL1和成骨细胞IL6蛋白和mRNA的表达皆显著降低。结论:滋阴补肾法能使骨髓基质细胞IL1和成骨细胞IL6表达降低,是其能够治疗骨质疏松症的机理之一。更多还原

【Abstract】 Objective:To observe the Effect of the method of nourishing the Kidney-Yin on the Expression of the bone matrix cell IL-1 and the osteoblast IL-6 in the Ovariectomy-induced Osteoporosis Rats,and to study the mechanism of the treating of the osteoporosis.Methods:Rats were randomly divided into five groups:the control,the pseudo-operation,the model,the estrogen and the nourishing the Kidney-Yin.After rats ovariectomized for three months,the rats were with the Zuogui pill for three months.The undecalcified sections in shankbone were measured by bone histomorphometry;the bone matrix cell IL-1 and the osteoblast IL-6 protein were detected by the immunohistochemistry,and the mRNA was detected by the method of in situ hybridization.Results:Compared with the model group,TBV% in shankbone of nourishing the Kidney-Yin rats heightened markedly,but TRS%,TFS%,MAR,OSW and mAR reduced markedly.At the same time,the expression of protein and mRNA of bone matrix cell IL-1 and osteoblast IL-6 all reduced markedly.Conclusions:The method of nourishing the Kidney-Yin can reduce both the expression of the bone matrix cell IL-1 and the osteoblast IL-6,and it is one of the mechanism to treat the osteoporosis.更多还原