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高效液相色谱-荧光检测法测定丹酚酸B的含量研究

Determination of Salvianolic acid B by High Performance Liquid Chromatography with Fluorescence Detection

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【作者】 王凤美李磊沈金灿王小如黎先春

【Author】 WANG Feng-mei~1,LI Lei~2,SHEN Jin-can~1,WANG Xiao-ru~2,LI Xian-chun~2(1.The Key Laboratory of Science of Ministry of Education of China,College ofChemistry and Chemical Engineering,Xiamen University,Xiamen 361005,Fujian,China;2.First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,Shandong,China)

【机构】 厦门大学化学化工学院化学系现代分析科学教育重点实验室国家海洋局第一海洋研究所国家海洋局第一海洋研究所 福建厦门361005山东青岛266061福建厦门361005山东青岛266061

【摘要】 建立了丹酚酸B的高效液相色谱-荧光检测含量测定方法。色谱条件为:BACKMANODS柱( 4 6mmi.d .×2 5 0mm ,5 μm) ,以甲醇-0 5 %冰醋酸水溶液为流动相,流速为1 0mL/min ;荧光检测波长λEX=3 3 8nm ,λEM=44 3nm ;进样量5 μL。丹酚酸B进样量为0 12~1 2 μg时,其质量与峰面积呈良好的线性关系(r=0 9998) ;加样回收率为98 6%~10 8% ;最低检测限为0 0 13 μg/mL。该法具有更高的灵敏度,为丹酚酸B的药代动力学研究提供基础

【Abstract】 A new method for the determination of Salvianolic acid B by high performance liquid chromatography with fluorescence detection has been established.The column used was BACKMAN C 18 (4.6mm i.d.×250mm,5μm);The mobile phase was methanol-0.5% acetic acid solution with gradient condition at a flow rate of 1.0mL/min;The fluorescence detector was operated at λ EX =338nm and λ EM =443nm with the injection volume of 5μL.The result proved that Salvianolic acid B has a good linearity in the range of 0.12μg to 1.2μg with the correlation coefficient of 0.9998;The recoveries were 98.6%~108%;The limit of detection is 0.013μg/mL.This method has a higher sensitivity and can be used in the study of pharmic metabolize.

【基金】 国家自然科学基金重点项目(2 0 2 350 2 0 )
  • 【文献出处】 中医药学刊 ,Study Journal of Traditional Chinese Medicine , 编辑部邮箱 ,2005年05期
  • 【分类号】R284.1
  • 【被引频次】12
  • 【下载频次】227
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