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高效液相色谱-荧光检测法测定丹酚酸B的含量研究
Determination of Salvianolic acid B by High Performance Liquid Chromatography with Fluorescence Detection
【摘要】 建立了丹酚酸B的高效液相色谱-荧光检测含量测定方法。色谱条件为:BACKMANODS柱( 4 6mmi.d .×2 5 0mm ,5 μm) ,以甲醇-0 5 %冰醋酸水溶液为流动相,流速为1 0mL/min ;荧光检测波长λEX=3 3 8nm ,λEM=44 3nm ;进样量5 μL。丹酚酸B进样量为0 12~1 2 μg时,其质量与峰面积呈良好的线性关系(r=0 9998) ;加样回收率为98 6%~10 8% ;最低检测限为0 0 13 μg/mL。该法具有更高的灵敏度,为丹酚酸B的药代动力学研究提供基础
【Abstract】 A new method for the determination of Salvianolic acid B by high performance liquid chromatography with fluorescence detection has been established.The column used was BACKMAN C 18 (4.6mm i.d.×250mm,5μm);The mobile phase was methanol-0.5% acetic acid solution with gradient condition at a flow rate of 1.0mL/min;The fluorescence detector was operated at λ EX =338nm and λ EM =443nm with the injection volume of 5μL.The result proved that Salvianolic acid B has a good linearity in the range of 0.12μg to 1.2μg with the correlation coefficient of 0.9998;The recoveries were 98.6%~108%;The limit of detection is 0.013μg/mL.This method has a higher sensitivity and can be used in the study of pharmic metabolize.
【Key words】 high performance liquid chromatography; fluorescence detection; salvianolic acid B;
- 【文献出处】 中医药学刊 ,Study Journal of Traditional Chinese Medicine , 编辑部邮箱 ,2005年05期
- 【分类号】R284.1
- 【被引频次】12
- 【下载频次】227