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香茶菜免疫活性成分的筛选研究

Study on Screening Immunocopetent Position in Rabdosia amethystoieds(Benth.) Hara

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【Author】 Chen Zijun~1,Li Yunsen~2,Zhou Jiyan~1,Cao Luye~3,Hu Yuejuan~1,Li Yikui~3(1.Institute of Chinese Meteria Medica of Shanghai University of Traditional Chinese Medicine,Shanghai 201203;2.Shanghai Instiute of Materia Medica,Shanghai 201203;3.The Experimental Center of Science and Technology of Shanghai University of Traditional Chinese Medicine,Shanghai 201203)

【机构】上海中医药大学中药研究所；中国科学院上海药物研究所；上海中医药大学科技实验中心；上海中医药大学科技实验中心上海201203；上海201203；上海201203；

【摘要】目的:以药理评价为导向,分离香茶菜中具有免疫活性的部位。方法:以有丝分裂原伴刀豆蛋白A(Co-nA)活化小鼠脾脏T淋巴细胞,采用噻唑盐(MTT)实验方法,体外检测香茶菜提取物对淋巴细胞增殖反应的影响,筛选具有免疫活性的有效部位。结果:香茶菜具有免疫增强作用的部位是FA、FC;而FB和FD则在不同浓度下有增强与抑制的不同作用。从FB中进一步分离得到的FB-1和FB-2以免疫增强作用为主,而FB-3和FB-4则以免疫抑制作用为主。结论:香茶菜中含有免疫活性成分。更多还原

【Abstract】 Objective: To search for immunocompetent position in Rabdosia amethystoieds(Benth.) Hara.Methods: Spleen cells of mice were stimulated by mitogen concannvalin A(ConA),and proliferation of lymphocytes were measured with tetrazolium salts(MTT) in vitro.With such immunopharmacological experiment,immunocompetent position of Rabdosia amethystoieds(Benth.) Hara directively were separated. Results: FA and FC could promote proliferation,but FB and FD had stimulative and inhibitory effect respectively at different concentration.FB-1 and FB-2 had promotive effect but FB-3 and FB-4 had suppressive effect mainly.Conclusion: There are immunocomponents in Rabdosia amethystoieds(Benth.) Hara.更多还原

【关键词】香茶菜；免疫药理； MTT试验；
【Key words】 Rabdosia amethystoieds(Benth.) Hara； Immunopharmacology； MTT assay；

【基金】国家科技部863计划“创新药物和中药现代化”新药博士基金资助课题(2003AA2Z3518)

【文献出处】中药材 ,Journal of Chinese Medicinal Materials , 编辑部邮箱 ,2005年11期

【分类号】R284.1;
【被引频次】15
【下载频次】323

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