【摘要】 目的:对大血藤不同器官和不同产地大血藤叶片提取物的抑菌活性进行了比较,为大血藤叶片的开发利用提供基础资料。方法:叶片60%丙酮提取物经石油醚和氯仿萃取后取水相作为试验药液,以金黄色葡葡球菌和枯草芽孢杆菌为实验菌株,以平板二倍稀释法统计最小抑菌浓度;以试管二倍稀释法统计最小杀菌浓度。结果:不同器官大血藤提取物的抑菌活性的大小顺序为叶片>饮片>多年生老茎>一年生嫩茎。对不同产地的大血藤叶片的抑菌活性大小顺序为大雷山>百山祖>华顶山>昌化>括苍山。结论:大血藤叶片有一定的抑菌活性,具有开发和利用的前景。更多还原

【Abstract】 Objective:To compare the anti-bacterial activity of the extracts from different organs and the leaves from different locations of Sargentodoxa cuneata,and to provide the basis for the exploitation of the leaves.Methods:The leaves was distilled with 60% acetone and the water phase after the extraction with petroleum ether and chroloform was used in the test.The Staphylococcus cureus and Pseudononas aerujinosa were used as the experimental strain.The minimal inhibition concentration was calculated using agar plate dilution method.The minimal bacterialcidal concentration was calculated using tube dilution method.Results:The anti-bacterial activity of the extraction of S.cuneata from leaves was the highest.That from slices took the second place and the third was that from old stems.That from twins was the lowest.The sequence of the anti-bacterial activity of S.cuneata from different locations was Daleishan>Bianshanzu>Huadingshan>Changhua>Kuochangshan.Conclusion:The anti-bacterial activity of the extraction from the leaves of S.cuneata can be detected and the foreground of the exploitation and utilization is good.更多还原