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多指标综合评分法优选西洋参提取工艺
Applying Grading Methods of Synthesizing Multiple Guidelines to Optimizing Extracting Technology for Radix Panacis Quinquefolii
【摘要】 目的:优选西洋参最佳提取工艺。方法:(1)采用正交试验法,以西洋参中人参皂苷Rg1、Re、Rb1的总转移率及浸膏得率为指标,多指标综合评分,优选西洋参的乙醇提取工艺条件;(2)运用RP-HPLC梯度洗脱法同时测定西洋参中的人参皂苷Rg1、Re、Rb1的含量。结果:西洋参乙醇提取的最佳条件为12倍量70%乙醇回流提取2次,每次提取3h。结论:该工艺可以有效提高西洋参的提取率。
【Abstract】 Objective: To optimize the best extracting procedure for Radix Panacis Quinquefolii. Methods: （1）The optimum alcohol extracting procedure was selected with the content and extraction efficiency of ginsenoside Rg1?Re?Rb1 from Radix Panacis Quinquefolii by orthogonal test design and synthesizing multiple guidelines method. （2）The content of three ginsenoides Rg1?Re and Rb1 in Radix Panacis Quinquefolii was determined by gradient elution method in RP-HPLC. Results: The optimum alcohol-extracting procedure was determined as 12 times of 70% alcohol refluxing and extracting 3h for 2 times. Conclusions: The optimum extracting procedure can increase extraction efficiency of Radix Panacis Quinquefolii.
【Key words】 Radix Panacis Quinquefolii； Ginsenoside Rg1、Re、Rb1； Orthogonal test design； Grading methods； Gradient elution；
- 【文献出处】 中药材 ,Journal of Chinese Medicinal Materials , 编辑部邮箱 ,2005年07期
- 【分类号】TQ461
- 【被引频次】20
- 【下载频次】685