【摘要】 目的:观察益骨胶囊预防和治疗用药对去卵巢骨质疏松大鼠骨组织TGF- β1mRNA基因表达的影响,在分子水平上阐明益骨胶囊防治骨质疏松症的可能作用机制。方法:从72只10月龄SD雌性大鼠中随机抽取24只为假手术A和B组,余48只待手术造模后随机分为4组:模型A组、模型B组、预防组、治疗组,每组12只;以双侧卵巢切除法复制骨质疏松模型;预防各组均在手术后3天即开始灌胃,假A组、模A组灌胃NS3ml、预防组灌胃益骨胶囊水溶液3ml,每天1次,共24周;治疗各组均在手术后第13周即开始灌胃,假B组、模B组灌胃NS3ml、治疗组灌胃益骨胶囊水溶液3ml,每天1次,共12周。FQ- PCR方法检测骨组织TGF- β1mRNA基因表达。结果:大鼠造模24周时,益骨胶囊预防组和治疗组骨组织TGF- β1mRNA基因表达均明显高于模型组,与假手术组相近。结论:益骨胶囊能促进骨组织TGF- βmRNA基因表达。更多还原

【Abstract】 Objective: To investigate the effects of Benefiting-bone capsule （BBC） on the expression of transforming growth factor-β₁ mRNA in bone tissue of the ovariectomized rats to further explain the mechanism of BBC on preventing and treating osteopoprosis. Methods: Twenty- four rats were randomly chosen from 72 female SD rats aged 10 months, which were classified into group A and B of sham operation dealing with normal physiological saline. The left 48 rats were randomly divided into 4 groups after ovariectomy: group A and B of osteoporotic animal model with normal physiological saline, prevented and treated groups of osteoporotic animal model with BBC. Each group included 12 rats. Ovariectomy was used to establish the osteoporotic animal model and the expression of TGF-β₁mRNA on gene level in bone tissue was detected by real time fluorescence quantitative PCR（FQ-PCR） method. Results: The expression of TGF-β₁mRNA on gene level in bone tissue of the groups of osteoporotic animal model with BBC were all higher than that of the groups of osteoporotic animal model, which were close to the groups of sham operation. Conclusion: BBC can improve the expression of TGF-β₁mRNA on gene level in bone tissue, which may be the mechanism of BBC on preventing and treating osteoporosis.更多还原