【摘要】 目的:研究家免口服苯妥英锌（PHTZ）对血清高密度脂蛋白胆固醇（HDL-C）的影响。方法:家兔分别口服PHTZ和苯妥英钠（PHTS）30d(剂量分别为:6,12,24mg·kg^-1·d^-1),用酶法测定血清中总胆固醇（TC）和三酰甘油（TG）的含量,HDL-C的测定采用磷钨酸镁盐沉淀后同TC测定法,低密度脂蛋白胆固醇（LDL-C）用费氏（Friedeuald）公式计算。用原子吸收分光光度法测定服药前后血清及服药后肝中Zn和Cu浓度。结果:PHTZ高、中剂量组分别使血清HDL-C升高76.0%（P<0.01）和52.1%（P<0.01）,LDL-C分别降低19.7%（P>0.05）和16.6%（P>0.05）,对TC和TG无显著影响。PHTZ使血清HDL-C升高的ED₅₀为12.7mg·kg^-1,PHTS使血清HDL-C提高的ED₅₀为23.7mg·kg^-1。PHTZ中剂量组使血清Zn浓度下降29.2%,与血清HDL-C上升的百分数呈负相关（r=-0.3035,P>0.05）,使肝脏Zn浓度上升56.2%,与血清HDL-C上升的百分数呈正相关（r=0.7263,P<0.05）,血清和肝脏中Cu无显著变化。结论:PHTZ使家兔血清HDL-C升高比PHTS更明显,ED₅₀比PHTS低,可能与分子结构中的Zn有关。更多还原

【Abstract】 Objective: To study the effects of phenytoin zinc （PHTZ） on serum high-density lipoprotein cholesterol （HDL-C） in rabbits. Methods: Rabbits were randomly dosed with either PHTZ or phenytoin sodium （PHTS） of 6,12 or 24mg·kg^1-·d^-1, respectively, for 30 days. The serum total cholesterol （TC） and triglycerides （TG） levels were measured with an enzyme assay.The serum HDL-C was precipitated from the supernatant with H₃P(W₃O₁₀)₄+MgCl₂ and then determined by the enzyme assay. The serum low-density lipoprotein cholesterol （LDL-C） level was derived from the Friedeuald formula. The zinc （Zn） and copper （Cu） levels in serum and liver were detected by atomic absorption spectrophotometry. Results: At 24 and 12 mg·kg^-1·d^-1, PHTZ significantly increased the HDL-C by 76. 0% （P<0.01） and 52.1% （P<0.01）, respectively, decreased the LDL-C by 19. 7% （P>0.05） and 16.6% （P>0. 05）, respectively, and remained unchanged in TC and TG levels. The PHTZ showed an ED₅₀ of 12.7mg·kg^-1 at the escalation of the HDL-C level and PHTS an ED₅₀ of 23. 7mg·kg^-1. 12mg·kg^-1·d^-1 PHTZ significantly reduced the serum Zn level by 29.2% with a negative correlation with the escalation of HDL-C level （r=-0. 303 5, P>0. 05）, and elevated the liver Zn level by 56.2% with a positive correlation with the escalation of HDL-C level （r=0. 7263, P<0.05）. PHTZ showed insignificant effects on the serum and liver Cu level. Conclusion: Compared with PHTS, PHTZ with a lower ED₅₀ showed a more powerful capacity in the escalation of HDL-C level, which might be associated with the existence of Zn.更多还原