【摘要】 目的:观察国产白细胞介素-1受体阻滞剂（IL-1ra）对大鼠胶原性关节炎（CIA）的影响。方法:鸡Ⅱ型胶原诱导大鼠CIA模型,关节评分法观察关节炎的发生情况;³H-TdR掺入法检测T细胞、B细胞增殖反应;采用小鼠淋巴细胞增殖法检测IL-1活性;放免法检测大鼠腹腔巨噬细胞（PMφ）培养上清液肿瘤坏死因子（TNF）和前列腺素E₂（PGE₂）的含量。结果:CIA大鼠在致炎后d10,出现关节红肿,刀豆蛋白（ConA）或脂多糖（LPS）诱导的T和B淋巴细胞增殖反应增强;PMφ产生IL-1,TNF-α,PGE₂的水平升高。IL-1ra(7.5,30,120mg·kg^-1·d^-1)和阳性对照组(anakinra 120mg·kg^-1·d^-1)sc,连续7d,均能减轻CIA大鼠关节局部炎症,降低关节评分;IL-1ra(30,120mg·kg^-1·d^-1)能明显降低CIA大鼠过高的T淋巴细胞增殖反应,IL-1ra(7.5,30,120mg·kg^-1·d^-1)能明显降低CIA大鼠B淋巴细胞增殖反应;降低CIA大鼠PMφ产生过高的IL-1,TNF-α及PGE₂水平。结论:IL-1ra对CIA大鼠淋巴细胞和巨噬细胞的增殖反应具有抑制作用。更多还原

【Abstract】 Objective:To investigate protections of interleukin-1 receptor antagonist （IL-lra） against collagen-induced arthritis （CIA） in rats. Methods: A CIA model was set up in SD rats by subcutaneous injection with type II collagen. The severity of arthritis was rated with arthritis scores. The examination of conA-and lipid polysaccharide （LPS）-induced splenocytes proliferation and interleukin-1 （IL-1） activities were conducted using a 3H-TdR intake method. Tumor necrosis factor alpha （TNF-α） and PGE₂ in the culture broth of macrophage （PMΦ） were quantified using radioimmunoassay. Results: The conA or LPS increased the level of T-and B-lymphocyte in 10 days after CIA in rats. The local inflammatory symptoms and B lymphocytes proliferation were reduced after subcutaneous injection with IL-lra at 7.5,30 and 120mg·kg^-1·d for 7 days, and T lymphocyte proliferation was suppressed when IL-lra at 30 and 120mg·kg^-1·d^-1 was s. c. given in rats with CIA. The IL-1, TNF-α and PGR₂ levels in the PMΦ were inhibited by IL-1ra. Conclusion: IL-1ra inhibits the proliferation of lymphocytes and macrophage in the CIA rats.更多还原