【摘要】 目的:通过序列比对分析SEN病毒(SENV)不同开放阅读框(ORF)基因系统进化情况。方法:用聚合酶链反应(PCR)方法扩增武汉地区SENVORF1和ORF2基因,结合GenBank中SEN病毒基因信息对该基因进行序列测定、并采用clutal软件系统进行分析。结果:武汉SEN病毒株与北京株同源性较高,与日本株有一定的差异,和TT病毒有较大差异。结论:SENV可能不是TTV亲缘关系较远的变异株,而是一群不完全相同成员组成的病毒种。更多还原

【Abstract】 Objective:To phylogenetic analysis of different open reading frame(OFR) of SEN virus. Methods:Polymerase reaction products of SENV were sequenced and phylognetic analysis with clutal computer soft according to sequences on Genebank. Results:The homololgy of nucleotide sequence between Wuhan isolates and Bejing isolate were semblable, had some difference between Japan isolate and much different with TT virus. [WTHZ]Conclusion:SEN virus, belong to the Circoviridae family, may be different strain from TT virus.更多还原