【摘要】 目的研究丙型肝炎病毒(HCV)1b型5’非编码区BamHⅠ酶切位点变异的区域性分布。方法对来自中国4个不同地区的64例HCV1b基因型血清样品进行5’-NCR扩增后,分别用MboⅠ、BamHⅠ限制性内切酶酶切分析,比较不同地区变异株分布的差异。结果测序证实中国HCV1b基因型在117位有BamHⅠ酶切位点。64例血清样品中,BamHⅠ单切点变异株,华南7例(28%),东北1例(10%),华北1例(11.1%),西南0例;MboⅠ及BamHⅠ切点均无的变异株,西南8例(40%),华南2例(4%),东北1例(10%),华北0例。结论证实HCV1b型BamHⅠ变异具有区域性分布特点,华南地区和西南地区变异较多,华北和东北变异较少。而这一变异是否与干扰素治疗耐药有关,尚有待于进一步研究。更多还原

【Abstract】 [Objectives] To investigate the epidemiological distribution of the mutants with BamHⅠ site of hepatitis C virus 1b isolated from four major regions of China. [Methods] 64 HCV genotype 1b samples were selected in four different areas of China. Then we amplified these samples from the 5′ noncoding region by RT-PCR and digested the products respectively with MboⅠ and BamHⅠ restriction enzymes. [Results] Of the 64 samples studied, the strains with one BamHⅠ restriction site were 7 (28%), 1 (10%), 0, 0, respectively from south, northeast, north and southwest of China, while the strains without MboⅠ or BamHⅠ site were 8, 2, 1, 0, respectively from southwest, south, northeast and north of China. [Conclusions] There are some regional distribution differences of the mutants with BamHⅠ site of hepatitis C virus 1b in China, which seemed that the mutants were more in the southern and southwestern regions while in northern and northeastern regions were less.更多还原