【摘要】 目的探讨白细胞介素1β(IL-1β)与地塞米松联合应用对人成骨细胞(HumanOsteoblast-likecellsHOS)芳香化酶活性的影响。方法对人的成骨细胞进行培养,设立实验组,分别加入IL-1β1ng/mL(实验Ⅰ组)、10ng/mL(实验Ⅱ组)、50ng/mL(实验Ⅲ组);分别加地塞米松浓度0.1uM(实验Ⅳ组)、1.0uM(实验Ⅴ组)、10.0uM(实验Ⅵ组);地塞米松浓度0.1uM和IL-1β1ng/mL(实验Ⅶ组);IL-1β10ng/mL和IL-1ra500ng/mL(实验Ⅷ组),对照组加培养液,采用氚水法检测芳香化酶活性。结果实验组Ⅰ,Ⅱ,Ⅲ组氚水放射强度与对照组比较放射强度明显增加,差异显著(P<0.05)。实验Ⅳ,Ⅴ,Ⅵ组放射强度与对照组比较有非常显著性差异(P<0.001)。实验Ⅶ组与对照组间差异无统计学意义.实验Ⅷ组放射强度高于对照组及实验组Ⅰ和Ⅳ,差异非常显著(P<0.001)。结论人成骨细胞中IL-1β、地塞米松分别可以增加芳香化酶的活性。IL-1β是通过与成骨细胞的IL-1受体相结合而起作用。地塞米松和IL-1β联合应用使芳香化酶活性增加,它们相互作用影响着骨骼中雌激素合成。更多还原

【Abstract】 Objective To study the stimulation of Interleukin-1β (IL-1β) and Dexamethasone on aromatase activity of human osteoblast-like cells (HOS). Methods In the present study, the effect of IL-1β and Dexamethasone on aromatase activity was investigated by measuring water released upon the conversion of androstenedione to estrone. The experimental group was divided into nine group: G1 (IL-1β 1 ng/ml), G2 (IL-1β 10 ng/ml), G3 (IL-1β 50 ng/ml), G4 (Dexamethesone 0.1 uM), G5 (Dexamethesone 1 uM), G6 (Dexamethesone 10 uM), G7 (Dexamethesone 0.1 uM and IL-1β 1 ng/ml), G8 (IL-1β 10 ng/ml and IL-1ra 500 ng/ml) and one control group. Results Administration of IL-1β or Dexamethasone increased aromatase activity by dose dependent manner. However, addition of IL-1β 1 ng/ml with Dexametheson 0.1uM resulted in 110% greater than control. Addition of 500 ng/ml of human recombinant IL-1 receptor antagonist (IL-1ra) neutralized the increased aromatase activity to control level. Conclusions These findings suggest that IL-1β and Dexamethasone stimulated the aromatase activity in osteoblast-like cells by promoting the estrogen synthesis and IL-1β regulates it through the IL-1 receptor. Meanwhile, the presence of Dexamethasone is necessary for the induction of aromatase. Both of them may be involved in the regulatory mechanism for estrogen formation in bone.更多还原