【摘要】 目的　研制具有抗感染作用的WO- 1生物衍生骨缓释材料。　方法　应用 型胶原和同种骨制备胶原生物衍生骨复合材料,将WO- 1吸附于胶原生物衍生骨构建成WO- 1生物衍生骨缓释材料,扫描电镜观察其形貌特征;将3H-四环素( 4 5 .1GBq/ mmol)以WO- 1标准品溶液稀释,吸附于胶原生物衍生骨,测定缓释材料中WO- 1的体外释放;将WO- 1生物衍生骨缓释材料置入接种金黄色葡萄球菌、大肠杆菌和绿脓杆菌的培养基中,检测其体外抑菌能力。将WO- 1生物衍生骨植入2 4只新西兰大白兔桡骨缺损处,术后9、16、2 3及30 d各处死2只兔测定血中WO- 1的浓度,以及材料周围肌肉与骨中的WO- 1的浓度。　结果　WO- 1生物衍生骨复合材料保留了天然骨的三维结构,表面有胶状膜覆盖;在体外释放实验中第1天呈暴发释放,以后呈恒定释放;对金黄色葡萄球菌、大肠杆菌等骨感染的常见致病菌有持久稳定的抑菌能力。体内实验中周围骨组织及肌肉组织中WO- 1在各时间点均保持较高的浓度,组间比较差异无统计学意义( P>0 .0 5 ) ,而动物血中WO- 1浓度较低,与骨及肌肉组织中WO- 1浓度相比,差异有统计学意义( P<0 .0 5 )。　结论　WO- 1生物衍生骨缓释材料具有良好的药物缓释功能及较强的抑菌能力更多还原

【Abstract】 Objective To develop a new tissue engineering bone material which has an anti-infective function. Methods Collagen loaded bio-derived bone material was made by using type-I collagen and allograft bone. WO-1 was absorbed to collagen loaded bio-derived bone, then the morphological feature of the new bone material was observed by scanning electronic microscopy. 3H-tetracycline was diluted by WO-1 solution, and was absorbed to collagen loaded bio-derived bone,then the releasing kinetics of WO 1 was detected by 3H tetracycline in vitro. WO-1 bio-derived bone material was grafted into a culture medium with staphylococcus aureus, escherichia coli, and pseudomonas aeruginosa to observe its bacteriostasis ability. WO 1 bio derived bone material was grafted into radius of defected rabbits, the concentration of WO 1 was detected on the 9th, 16th, 23th, and 30th day by HLPC in blood, in bone and in muscle. The bacteriostasis ability of WO 1 loaded bio derived bone was tested in vitro and in vivo. Results WO 1 loaded bio derived bone maintained natural network pore system and the surface of network pore system was coated with collagen membrane. The release of WO 1 from WO 1 loaded bio derived bone showed bursting release on the 1st day, then showed stable release. WO 1 loaded bio derived bone showed lasting and stable bacteriostasis to common pathogens of orthopaedic infections. The high concentration of WO 1 was observed in bone tissue and in muscle tissue at different time points and the difference among groups had no significance( P >0.05), while the concentration of WO 1 in blood was very low( P <0.05). Conclusion WO-1 loaded bio-derived bone has good capability of drug controlled-release and bacteriostasis.更多还原