The formation of the polarity of Lilium davidii Duch pollen protoplast and the dynamics of actin cytoskeleton were observed by non-fixation, Alex-phalloidin probing and confocal laser scanning microscopy. The results showed that the protoplast obtained from stored pollen contained the numerous crystalline fusiform bodies to constitute a storage form of actin. When stored pollen was hydrated, the actin cytoskeleton in the protoplast formed a fine network spreading uniformly. In the process of polarity format...