【摘要】 目的:探讨神经元型一氧化氮合酶(nNO S)在创伤性脑损伤(TB I)中的作用。方法:采用落体法大鼠TB I动物模型,观察特异性nNO S抑制剂7-硝基吲唑(7-N I)对TB I早期脑水肿和病理变化的影响。结果:与伤后6 h组相比,7-N I能显著减少脑组织含水量和脑组织中N a+的含量(P值均<0.05),而增加K+的含量(P<0.05),并能改善脑创伤后的病理变化。7-N I的作用可被L-精氨酸逆转,D-精氨酸无效。结论:nNO S来源的一氧化氮对TB I早期起毒性作用,nNO S抑制剂有可能成为治疗继发性脑损伤的新途径。更多还原

【Abstract】 Objective:To investigate the function of neuronal nitric oxide synthase(nNOS) in traumatric brain injury(TBI).Methods:Using a rat model of focal cortical contusion according to Feeney,we studied the effect of nNOS inhibitor 7-nitroindazole(7-NI) on early traumatric cerebral edema and pathologicchange in cerebral cortex.Results:Comparing with 6 hours after TBI group,administration of 7-NI significantly mitigated traumatric cerebral edema,and decreased the content of Na~+ within brain(P<0.05,P<0.05,respectively),and increased the content of K~+(P<0.05).Histological examination showed that cortical contusion was improved treated with 7-NI.The effect of 7-NI was reversed by coadminstration of L-arginine,but not D arginine.Conclusion:Nitric oxide derived from nNOS may act as a neurotoxic mediator in early traumatric brain injury.nNOS inhibitors may be used to treat secondary brain injury in future.更多还原