【摘要】 目的探讨乙型肝炎病毒前S1蛋白(preS1)与乙型肝炎病毒血清标志物(HBV-M)以及HBV-DNA之间的关系;了解preS1在乙肝病毒感染及复制中的应用价值。方法应用ELISA法检测1254例preS1和HBV-M;应用荧光定量PCR法检测372例HBV-DNA。结果1254例中有573例preS1阳性,阳性率45.7%并且preS1阳性只存在于HBsAgHBeAgHBcAb(大三阳)、HBsAgHBeAbHBcAb(小三阳)、HBsAgHBcAb(小二阳)3种血清模式中,阳性率分别为96.0%、68.6%、81.4%,经χ2检验,3者之间差异极显著。分析372例HBV-DNA,有292例>1×104,其中preS1阳性268例,阳性符合率为91.8%,而HBeAg阳性符合率为53.4%;另外80例HBV-DNA<1×104病例中,preS1阳性率仍为50.0%,而HBeAg阳性率仅为2.5%。结论通过对preS1、HBeAg和HBV-DNA的检测分析,证实了preS1作为判断HBV病毒感染和复制的指标比单纯用HBeAg和HBV-DNA定量更敏感,更确切,更方便,是一项非常好的检测指标。更多还原

【Abstract】 Objective:To study the relationship between preS1 antigen and HBV-markers and HBV-DNA.and to Understand the clinical values of preS1 in detecting HBV infection and replication.Methods:PreS1 antigen and different HBV-markers from 1254 serum specimens with HBV were determined by ELISA;372 serum specimens of HBV-DNA were tested by FQ-PCR.Results:PreS1 postive rates of 1254 serum specimens were 45.7%,and they were 96.0%,~68.6% and 81.4% respectively only among the three models of HBsAg HBcAg HBcAb,HBsAg HBeAg HBcAb and HBsAg HBcAb,and the differences of postive rates among them were significant by.χ2-test(χ2=18.14,13.14,12.02).There were 292 of postive HBV-DNA(>1×104 copy/ml) in 372 cases HBV-DNA anaylzed.PreS1 postive rates were 91.8%(268/292),but HBeAg postive rates were 53.4%.The postive rates of preS1 and HBeAg were 50.0% and 2.5% respectively in 80 HBV-DNA[<1×104) copy/ml].Conclusions:By detecting and analysis of preS1,HBV-M and HBV-DNA,it could be concluded that preS1 could be used as a more senstive, more simple and more exact marker for early diagnosis and reflecting HBV-DNA replication compared with only quantitative determination of HBeAg and HBV-DNA level.It should be the best marker of diagnosis of HBV.更多还原