【摘要】 50 mg/L 的硒胁迫下,小球藻中β- 胡萝卜素、类叶黄素、叶绿素(叶绿素a、叶绿素b)的含量先明显增加,之后逐渐下降;而在800 mg/L硒胁迫下,各种光合色素含量均明显下降;小球藻活体细胞在693 nm处出现叶绿素a 的吸收峰,其吸收值在硒胁迫后明显减弱;在室温荧光发射光谱中,700 nm处的发射峰随着硒浓度的增加而显著下降;荧光激发光谱表明:硒胁迫使小球藻的能量传递受阻,传递效率降低;藻体中水溶性蛋白含量随着硒胁迫的增强而下降。等离子体原子发射光谱(ICP-AES)研究结果表明:随着硒浓度增加,藻体中Mg2+、Ca2+、K+ 和Na+ 含量呈明显降低趋势,而培养液中这些离子的浓度则不断增加。更多还原

【Abstract】 Changes in photosynthetic pigment and pro-tein contents, growth and the spectral characteristicsin Chlorella vulgaris in response to selenium stresswere investigated. Carotenoids (β-carotene andxanthophylls) and chlorophyll (Chl a and Chl b) con-tents in cells exposed to Se 50 mg/L increased prima-rily and decreased afterwards, while photosyntheticpigments in cells exposed to Se 800 mg/L decreasedsignificantly. Chlorophyll (Chl) absorption peak at 693nm and prominent Chl emission peak at 700 nm, weak-ened significantly after Se stress. The excitation spec-tra showed a decrease in excitation energy transfer ef-ficiency in Se-stressed cells. Total soluble protein de-creased after Se stress. The changes in total Se, Mg2+,Ca2+, K+ and Na+ concentrations in culture medium andcells were also determined by ICP-AES.更多还原