【摘要】 目的:分析辽宁省不同城市和大连地区2000~2004年伤寒沙门菌的同源性以及在遗传学上的关系。方法:实验采用EcoR I/M seI型限制性内切酶、选择7种引物组合对25株不同地区和不同时间的伤寒沙门菌菌株进行AFLP分型。结果:25株伤寒沙门菌分为16个基因类型,不同来源的菌株呈现多态性分布;大连地区不同年份的15株菌存在多达14个多态型性;而其他3个城市共10株菌仅存在2个多态性,且遗传距离非常相近。结论:AFLP可以通过对内切酶组合的选择和特异性引物上选择性碱基组合的设计优化实验条件;AFLP是伤寒沙门菌的分子流行病学研究中一种十分有效的手段。更多还原

【Abstract】 Objective:To analyze intraspecific genetic relationships among Salmonella typhi strains isolated from diverse geographic areas in Liaoning province during 2000~2004.Methods:Amplified fragment length polymorphism (AFLP) was applied to 25 Salmonella typhi isolates using the restriction enzymes EcoRI/MseI and 7 primer pair combinations.Results:25 different Salmonella strains comprising 16 gene types were genetically identified by AFLP (ecmg primer pair combination).15 Salmonella typhi isolated from diverse years in Dalian city comprise 14 gene types; 10 strains isolated from other three cities comprise 2 gene types.Conclusion:[WTBZ]AFLP can be optimized by adjusting combinations of the restriction enzymes and the primer pair.AFLP is an effective method in studying on the characteristics of molecular epidemiology for Salmonella typhi.更多还原