【摘要】 目的:建立保健油脂中多不饱和脂肪酸及角鲨烯的GC/MS分析方法。方法:多不饱和脂肪酸以KOH-甲醇法进行甲酯化,用异辛烷提取多不饱和脂肪酸甲酯,经HP-FFAP石英毛细管柱分离,用气相色谱-质谱联用仪(GC-MS)进行定性?定量分析。结果:5种多不饱和脂肪酸及角鲨烯的标准溶液浓度在0.025~0.40 mg/m l范围内线性关系良好,相关系数r>0.9990;加标平均回收率范围为94.9%~101%;相对标准偏差(RSD)<5%。结论:本方法简便快速,准确可靠,适用于保健油脂中多不饱和脂肪酸及角鲨烯的检测。更多还原

【Abstract】 Objective:To develop a method for analyzing the multi-unsaturated fatty acids in healthy oils by GC/MS.Methods:The multi-unsaturated fatty acids was fist methylated with potassium hydroxide-methanol(KOH-MeOH), extracted by iso- octane, isolated by HP-FFAP silica capillary column, identified and determined by gas chromatograph equipped with mass spectrometer.Results:The calibration curves were linear within the ranges of 0.025～0.40 mg/ml for all standard solution, relative coefficient was over 0.9990;The average recoveries ranged from 94.9% to 101%; Precisions (RSD) were under 5% for all the analyzed components.Conclusion:The method was simple, rapid, accurate, and suitable for the determination of mulit-unsaturated fatty acids in healthy oils.更多还原