【摘要】 目的从微生物学角度寻找食源性疾病的病因。方法采用食品卫生微生物检验国家标准(GB/T4789),霍乱诊断标准及处理原则(GB15984-1995),结合mini-VIDAS全自动荧光酶标免疫测试仪和VITEK120全自动微生物分析仪对可疑食品进行食源性致病菌检测。结果从面包样品中直接检出葡萄球菌肠毒素;且面包检出产肠毒素的金黄色葡萄球菌;大多数面包细菌总数和大肠菌群超标。药敏试验显示,分离到的金黄色葡萄球菌对青霉素G、头孢他定、四环素、利福平耐药。结论结合流行病学调查、临床表现和微生物学检测结果,确认这起食源性疾病是由产生肠毒素的金黄色葡萄球菌污染面包所致。更多还原

【Abstract】 Objective:To search for the pathogen of a food-borne disease from the perspective of microbiology.Methods:The suspected pure milk and bread were tested to determine whether they were contaminated with pathogenic bacteria using the national standards microbiological examination of food hygiene(GB/T4789)and cholera diagnostic standards and managing principles(GB15984-1995)and mini-VIDAS apparatus and VITEK120 automated microbial system were utilized in the process.Results:Staphylococcal enterotoxin was detected directly from two bread samples. Two enterotoxigenic strains of Staphylococcus aureus were detected from the same two bread samples but the enterotoxin produced by the two strains could′t be typed.The aerobic bacterial count and coliform bacteria in most bread were above normal values.Antimicrobial drug resistance test showed that the two Staphylococcus aureus isolates were resistant to penicillin G, ceftazidime,tetracyclin and rifampicin.Conclusion:Microbiological examination,together with epidemiological investigation and clinical syndrome, showed that this food-borne disease was caused by of Staphylococcus aureus. [更多还原