【摘要】 本研究以9个含不同叶霉病抗病基因的番茄品种为试材,通过接种鉴定表明,Cf-5、Cf-9、Cf-11和Cf-19基因对我国目前的2个叶霉菌优势生理小种均具有较强的抗性。根据Cf-9基因设计引物,扩增Cf-9基因的片段,含Cf-9、Cf-11和Cf-19基因的3种番茄均获得了2·7kb的扩增片段。但用限制性内切酶TaqⅠ对PCR产物酶切可以将3种材料明显区分开来,Cf-9的2个差异酶切片段为1170和460bp;Cf-11的2个差异酶切片段为1100和410bp;Cf-19的2个差异酶切片段为1210和300bp,从而建立了3个基因的分子标记。在F2分离群体中验证表明,3个基因的分子标记鉴定结果与抗性接种鉴定结果是一致的,用这些标记可以进行分子标记辅助选择。更多还原

【Abstract】 In this paper, the resistant profiles of 9 tomato varieties with different resistant genes to tomato leaf mould caused by Cladosporium fulvum were analyzed by inoculating with two predominant physiological races at seedling stage. The result showed that the cultivars containing resistant genes Cf-5,Cf-9,Cf-11 and Cf-19 were highly resistant to above races. Polymerase chain reaction (PCR) with a primer pair based on the Cf-9 gene sequence generated a 2.7 kb fragment from all the hosts containing Cf-9,Cf-11 and Cf-19 genes. Further restriction fragment length polymorphism (RFLP) analysis with restriction enzyme Taq I showed more than three fragments with different pattern from each PCR product, in which the fragments, 1 170 and 460 bp from Cf-9, 1 100 and 410 bp from Cf-11, 1 210 and 300 bp from Cf-19, could be separately identify each of the three resistant genes. Genetic analysis of tomato F 2 progeny confirmed that the PCR-RFLP-based markers were closely linked to the resistant phenotype. Those findings strongly indicate that the PCR in combination with Taq I restriction digestion is a useful tool for resistant gene identification in tomato breeding.更多还原